- 作者: 邱式鴻 ; 吳世雄
- 作者服務機構: 台灣大學生化科學研究所
- 中文摘要: This account describes work in our laboratories on the application of physical methods to the structural studies of various toxins during the past few years. A general review of background and the meaningful results obtained from these approaches are described. The results are compared with updated information on related subjects carried out at other laboratories. Examples of structural studies on some small toxins present abundantly in Formosan cobra (Naja naja atra), one of the indigenous toxic snakes in Taiwan, are given with the emphasis on the identification and characterization of complex toxic protein components based on near-infrared Fourier transform Raman Spectroscopy. We will also describe our initial efforts in solving the solution structures of several small proteins and peptides by means of two-dimensional nuclear magnetic resonance (2D-NMR) and computer-simulated modeling. The structural information obtained by these modern physical techniques provides the framework for unraveling the complex structure-activity relationships engendered by these biomolecules.
- 英文摘要: --
- 中文關鍵字: Two Dimensional Nuclear Magnetic Resonance (2D Nmr); Near Infrared Fourier Transform Raman Spectroscopy; Snake Toxin; Phospholipase; Cobrotoxin; Cardiotoxin; Pro
- 英文關鍵字: 二級核磁共振;近紅外線傅立葉轉換拉曼光譜;蛇毒蛋白;磷脂;結晶毒蛋白;心臟毒素;蛋白質工程