- 作者: Tsu-Chung Chang; Wei-Yuan Chou; Gu-Gang Chang
- 作者服務機構: Department of Biochemistry, National Defense Mediacal Center, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: All biomacromolecules are faced with oxidative stress.Oxidation of a protein molecule always induces inactiva-tion of the molecule and introduces a tag to that mole-cule. These modified protein molecules are prone to deg-radation in vivo by the proteasome system. Couplingof protein modification and degradation of chemicallymodified proteins is one of the normal protein turnoverpathways in vivo. We call this a'chemical apoptosis' pro-cess, which is one of the early manifestations of pro-grammed cell death. Impairment of the proteasome sys-temleads to accumulation of modified nonfunctionalproteins or'aged proteins'that might cause various clini-cal syndromes including cataractogenesis, prematureaging, neurological degeneration and rheumatoid dis-ease. The metal-catalyzed oxidation of biomacromole-cules provides an excellent artificial aging system invitro. The system is very useful in the characterization ofstructure and function relationships of proteins (en-zymes), especially in those containing metal bindingdomain(s), because the oxidation is always followed byan affinity cleavage at the metal binding site(s) thatallows easy identification and further characterization.
- 中文關鍵字: --
- 英文關鍵字: --