- 作者: Sarah L. Milton; Gauri Nayak; Peter L. Lutz; Howard M. Prentice
- 作者服務機構: 1 Department of Biological Sciences SC 281, Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, USA; 2 Department of Biomedical Sciences, Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, USA
- 中文摘要: --
- 英文摘要: Neuroglobin is a heme protein expressed in the vertebrate brain in mammals, fishes, and birds. The physiological role of neuroglobin is not completely understood but possibilities include serving as an intracellular oxygen-carrier or oxygen-sensor, as a terminal oxidase to regenerate NAD+ under anaerobic conditions, or involvement in NO or ROS metabolism. As the vertebrate nervous system is particularly sensitive to hypoxia, an intracellular protein that helps sustain cellular respiration would aid hypoxic survival. However, the regulation of Neuroglobin (Ngb) under conditions of varying oxygen is controversial. This study examines the regulation of Ngb in an anoxia-tolerant vertebrate under conditions of hypoxia and anoxia. The freshwater turtle Trachemys scripta can withstand complete anoxia for days, and adaptations that permit neuronal survival have been extensively examined. Turtle neuroglobin specific primers were employed in RT-PCR for determining the regulation of neuroglobin mRNA expression in turtles placed in normoxia, hypoxia (4 h), anoxia (1 and 4 h), and anoxia-reoxygenation. Whole brain expression of neuroglobin is strongly upregulated by hypoxia and post-anoxic-reoxygenation in T. scripta, with a lesser degree of upregulation at 1 and 4 h anoxia. Our data implicate neurglobin in mediating brain anoxic survival.
- 中文關鍵字: --
- 英文關鍵字: anoxia, hypoxia, neuroglobin, Trachemys scripta