第5卷‧第2期,
198104
, pp. 111-118
快速鑑定腸內桿菌之多管微量系統
- 作者：
蔡文城；陳正言；鄭德?
- 作者服務機構：
國立陽明醫學院微生物學科; 榮民總醫院傳染病科
- 中文摘要：
我們研究室所設計的ERA-24E?鑑定腸內桿菌科菌種之微管多項試驗系統。本系統含有24種生化試驗，每一實驗操作 於塑膠盤中之個?微管，然後根據生化試驗正負反應結果的形式，計算出一個八位數的密碼，細菌的最後鑑定則依據針對 ERA-24E所設計的電腦密碼系統。藉著與傳統大管試驗相比較，來評估ERA-24E系統的有效性（生化試驗之一致性及 細菌鑑定的正確性）。在總共6，216個生化試驗中，兩系統之一致性達96. 6%；而在259個細菌中，正確性達96.5%，另 外3.1%屬於非常符合，即僅須一或兩個特性就可做正確鑑定。因此，ERA-24E系統?一種簡單且經濟的方法，能快速 且正確地鑑定出腸內桿菌，?臨床微生物檢驗室有效的鑑定工具。
- 英文摘要：
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- 中文關鍵字：
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- 英文關鍵字：
ERA-24E is a new micro-tube, multi-test system designed by our laboratories for the identification of Enterobacteriaceae within 18-24 hours. It consists of 24 biochemical tests. Each test is in its own micro-tube in a polystyrene tray. Based on the pattern of positive and negative biochemical test results, an eight-digit octal code number is calculated. Bacterial identification is based on a computer-generated coding system. The effectiveness of the ERA-24E system was evaluated with respect to its agreement in biochemical tests and accuracy in bacterial identification by comparing it with the conventional macro-tube method. Of the 9,216 biochemical tests performed, there was 96.6% agreement between the ERA-24E and conventional systems. Of the 259 organisms tested, 96.5% were identified correctly l-y the ERA-24E system, and 3.1% were highly compatible, i.e. required one or two test(s) for differentiation. The ERA-24E system was found to be a simple, economic method for rapid, and accurate identification of the Enterobacteriaceae.
