- 作者: 羅錦榮,許瑋琪,鍾自強,曾慶傑,李國明,伍俊明
- 作者服務機構: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, P. R. China
- 中文摘要: In view of the fact that many transition metal complexes display intense and long-lived metal-to-ligand charge-transfer emission with a large Stokes’ shift, we have incorporated biotin into a series of luminescent rhenium(I), iridium(III) and ruthenium(II) polypyridine complexes to form new probes for the protein avidin. All these luminescent transition metal polypyridine biotin complexes displayed enhanced emission intensities and extended lifetimes upon binding to avidin. These changes are closely related to the hydrophobic biotin-binding pockets of avidin and the increased rigidity of the local environment of the probes after they bind to the protein. We have exploited the characteristics of these luminescent transition metal biotin complexes in the development of various assays.
- 英文摘要: --
- 中文關鍵字: Biotin; Iridium(III); Luminescence; Rhenium(I); Ruthenium(II).
- 英文關鍵字: --