- 作者: Meng-Ing Chou; Yu-Fan Hsieh; Meilin Wang; Jinghua Tsai Chang; Deching Chang; Moncef Zouali; Gregory J Tsay
- 作者服務機構: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background: RNA interference (RNAi) is a powerful tool to silence gene
expression post-transcriptionally. Delivering sequences of RNAi in vivo
remains a problem. The aim of this study was to use JC virus (JCV) virus-like
particles (VLPs) as a vector for delivering RNAi in silencing the cytokine gene
of IL-10.
Methods: JCV VLPs were generated by recombinant JCV VP1 protein in
yeast expression system. DNA fragment containing IL-10 shRNA was
packaged into VLPs by osmotic shock.
Results: In RAW 264.7 cells, IL-10 shRNA was found to reduce IL-10
expression by 85 to 89%, as compared with VLPs alone. IL-10 shRNA did not
cross-react with TNF-alpha mRNA or influence the expression of TNF-alpha.
In BALB/c mice IL-10 shRNA could reduce 95% of IL-10 secretion. Surprisingly,
it also down regulated TNF-alpha expression.
Conclusions: We show for the first time that JCV VLPs empty capsids are
competent vectors to deliver RNAi and are nontoxic to cells, suggesting that
JCV VLPs is an efficient agent to deliver RNAi in both murine macrophage
cells and BALB/c mice. This system provides an efficient means for delivering
the RNAi for gene therapy purposes. - 中文關鍵字: --
- 英文關鍵字: --