• 作者： 王三郎; 白其昇; 謝順堂
• 作者服務機構： 大葉工學院食品工程系
• 中文摘要： Pseudomonas aeruginosa M-1001能同時產生雞蛋白溶解酵素抑制劑及胞溶酵素於培養液，但所生產之胞溶酵素不受此抑制劑所抑制。於含0.55％ glucose，0.75％ soluble starch，0.25％ beef extract，0.25％ polypepton，0.25％ sodiumL-glutamate，以及0.0001％ NaCI (pH 6)之培養基中，經36小時培養(37C)時可得較佳之胞溶酵素活性。酵素反應之pH及熱穩定性分別為pH 6-8及40C。此菌株所生產之胞溶酵素對多數測試菌株以及Saccharomyces cerevisiae之生長具抑制作用，而對其本身以及其他少數細菌則無抑制效果。
• 英文摘要： Pseudomonas aeruginosa M-1001 produced both hen-egg-white lysozyme inhibitors and lytic enzymein the culture broth. The lytic enzyme produced was not inhibited by the lysozyme inhibitors. Maximallytic activity was obtained when the strain was grown aerobically in a medium consisting of 0.25% glucose,0.75% soluble strach, 0.25% beef extract, 0.25% Polypepton, 0.25% sodium L-glutamate and 0.0001%NaCI (pH 6), at 37C after 36 hrs. The lytic enzyme was stable at pH from 6 to 8 and temperatures below40C. Many bacteria and Saccharomyces cerevisiae were found to be inhibited by the crude lytic enzymeproduced from the M-1001 strain except for the strain itself and a few other microorganisms.
• 中文關鍵字： lytic enzyme; autolysin; lysozyme; lysozyme inhibitor; antibiocti; Pseudomonas aeruginosa.
• 英文關鍵字： --