• 作者： 陳守仕; 蘇杰夫; 林榮福; 廖述吉; 鄭建盛; 張瑞森; 劉正義
• 作者服務機構： 臺灣省家畜衛生試驗所; 農林廳畜牧科; 臺灣省立屏東農業專科學校
• 中文摘要： 約由39,000頭乳牛經結核菌素(Tuberculin)檢驗呈陽性反應之撲殺牛隻68頭，取材進行病原菌之分離。經以 Herrold's 培養基分離培養，共得28例菌株，並續行多種生化性狀及實驗動物之人工感染試驗，以鑑定目前臺灣乳牛所感染結核菌病之菌型。分離株顯示對 Thiophen-2carboxylic acid hydrazid, Isonicotinic acid hydrazid, Neotetrazolium chloride, Streptomycin,Rifampin 有感受性，而 Niacin 呈陰性反應，以及分離株對天竺鼠、家兔皆有感染性，乃同被定為Mycobacterium bovis o此次試驗證明目前臺灣乳牛所感染之結核病乃屬牛型結核菌。
• 英文摘要： About 39,000 dairy cattle tested withmammalian tuberculin, 68 cattle showed positivereaction, then, they were slaughtered andsampled for Mycobacterium (M.) isolation andpathological examination. Twenty-eight out of68 cases were isolated by using Herrold'smedium. These isolates were characterized bytheir biochemical properties and pathogenicities.All the isolates showed sensitive to isonicotinicacid hydrazid, thiophen-2-carboxylic hydrazid,neotetrazolium chloride, streptomycin andrifampin, but resistant to niacin test. Theseorganisms were pathogenic guinea pigs andrabbis, and nonpathogenic to chicken. Therefore,the isolated strains all belonged to M. boviswhich are considered as the main cause oftuberculosis in Taiwan.
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