- 作者: Chien-Wen Chen; Tzu-Yang Lin; Tsan-Chi Chen; Jyh-Lyh Juang
- 作者服務機構: 1 Division of Molecular and Genomic Medicine, National Health Research Institutes, 35, Keyan Road, Zhunan Town, Miaoli County 350, Taiwan; ; 2 Department of Life Science and Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan
- 中文摘要: --
- 英文摘要: Translation efficiency is often regulated in part by 5'-untranslated region (5'UTR). Sequence analysis of an evolutionarily conserved stress-responsive protein, Drosophila Peroxiredoxin I (dPrx I), found the transcript to have two alternative 5'UTRs that lead to an identical coding sequence: namely Ia and Ib. Although both isoforms coexisted in Drosophila cells, the Ia isoform appeared to be dominant. Furthermore, reporter assay found that Ia enhanced translation in steady-state cells while Ib increased translation in cells under oxidative stress. Together, our data suggest that the two alternative 5'UTRs of dPrx I may be involved in a translational regulatory mechanism that responds to cellular oxidative stress.
- 中文關鍵字: --
- 英文關鍵字: alternative splicing, Drosophila, IRES, 5'UTR, Prx I, stress-response, translation enhancement