- 作者: S. Kaushal ; V.F. La Russa ; E.R. Hall ; S. Gartner ; J.H. Kim ; LP. Perera ; Z. Yu; S. W. Kessler ; J.D. Mosca
- 作者服務機構: a Henry M. Jackson Foundation for the Advancement of Military Medicine and Walter Reed Army Institute of Research, Rockville, Md., ; b Bone Marrow Transplantation Program,Tulane Cancer Center, New Orleans, La., ; c The Johns Hopkins Hospital,Department of Neurology, ; d Institute for Human Virology,University of Maryland at Baltimore and ; e Osiris Therapeutics, Baltimore, Md., ; f National Institute of Allergy and Infectious Disease, National Institutes of Health,Bethesda, Md., and ; g Systemix, Palo Alto, Calif., USA; ; h US Naval Medical Research Unit 3, Cairo, Egypt
- 中文摘要: --
- 英文摘要: In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraft-ment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.
- 中文關鍵字: --
- 英文關鍵字: SCID mice ; Hematopoiesis ; Stromal cells ; CD34+ cells ; Microenvironment