• 作者： 翟建富; 任瑞芝
• 作者服務機構： 國立陽明醫學院生化研究所
• 中文摘要： 蘇力菌亞種aizawai HD133中的一個cry基因的序列已被定出。此基因能產生一個含有1155個氨基酸的蛋白，其分子量是130622 Da。此序列與蘇力菌亞種aizawai IPL7和berliner 1715中的cry基因序列相比時，發現彼此間只有五個核甘酸相異，所以此cry基因應屬crylA(b)類。開放閱讀框上游的72個核甘酸序列已足夠策動此基因在大腸桿菌中表現的能力。利用一放動子探針質體，我們發現任何帶有這72個核甘酸序列的DNA片段都顯示有啟動子的活性，很可能這一個72核甘酸序列含有一個可在大腸桿菌中表現的奇異啟動子。
• 英文摘要： The sequence of a cry gene from Bacillus thuringiensis var. aizawai HD133 was determined. Thiscry gene encodes a protein of 1155 amino acids, the molecular weight of which is 130622 Da. When thenucleotide sequence of this cry gene was compared with the nucleotide sequence from B. t. var. awzawaiIPL7 and var. berliner 1715，only five nucleotide changes were found. Therefore, this cry gene should begrouped into the crylA(b) gene type. A 72 nucleotide sequence upstream of the open reading frame of thecry gene was enough to conduct the transcription of the cry gene in E. coli. Using a promoter probe vectorsystem, it was demonstrated that any DNA fragment containing this 72 nt sequence exhibited promoteractivity in vivo. It is likely that a putative promoter sequence may be present within this 72 nucleotidesregion for the expression of this cry gene in E. coli.
• 中文關鍵字： Bacillus thuringiensis; nucleotide sequence; promoter activity; cry gene expression.
• 英文關鍵字： --