- 作者: 周松男; 吳志正; 黃思誠; 蔡育倫; 簡靜香
- 作者服務機構: Department of Obstetrics and Gynecology; National Taiwan University Hospital; Department of Obsterics and Gynecology; Provincial Tao-Yuan Hospital; Department of Biochemistry; Naitional Yang-Ming Medical College
- 中文摘要: 本研究對象包括40位良性卵巢腫瘤及25位卵巢癌病患,在手術前採血,分別以血液生化自動分析儀(Hitachi-736),電泳分離及密度測定儀測定血清中乳酸脫氫酵素濃度及其異型酵素型態。LDH血清總量在卵巢癌病患平均是876.3IU/1,遠高於良性卵巢腫瘤的364.8IU/1,有22例卵巢癌(88%)及6例良性腫瘤(15%)高於正常值450IU/1。在異型酵素的分析上,卵巢癌病患血清中LDH-4與LDH-5異常增高(分別佔9.5%及14.6%)。對診斷卵巢惡性腫瘤,LDH值的增高可提供88%及85%的敏感性與特異性,而LDH-4與LDH-5的異常增高可提供84%與77.5%的敏感性及特異性。若同時分析LDH血清總量及其異型酵素,則對診斷卵巢癌的陽性預測值及陰性預測值幾近100%。 本研究顯示同時分析病患血清中LDH總量與其異型酵素型態有助於卵巢癌的診斷。
- 英文摘要: A total of 65 cases of ovarian tumor admitted for surgical intervention were prospectively enrolledin this study, including 40 cases with benign ovarian tumors and 25 cases of ovarian cancer. The 25 mali-gnancies were comprised of 22 cases of common epithelial origin and three cases with Krukenberg tumors. Serum total LDH level and its isozyme activity were measured preoperatively in each patient. Themean value (±SD) of serum LDH in the malignant group was 876.3 (±450.4) IU/1, which was significantlyhigher than 364.8 (±87.9) IU/1 in the benign group. Twenty-two cases of ovarian malignancy (88%) and 6 cases of the benign group (15%) had elevatedserum LDH levels above 450 IU/1. Analysis of the LDH isozyme pattern demonstrated that there wasa significant shift to LDH-4 and LDH-5 fractions in ovarian malignancy when compared to the benigngroup (with the mean value of 9.5%, 14.6% vs. 6.5% and 4.6%, respectively). The elevated LDH level couldyield 88% and 85% in sensitivity and specificity for detecting ovarian malignancy, while the abnormalisozyme pattern could yield 84% and 77.5%, respectively. From combined analysis of total LDH levelsand isozyme patterns, the true positive detection rate and true negative detection rate could both reach100%. In conclusion, the present findings imply that total serum LDH level and isozyme acti-vity measurement may provide significant aids in evaluation and detection of human ovarian cancers amongpatients with ovarian tumors.
- 中文關鍵字: lactic dehydrogenase; isozymes; ovarian neoplasms.
- 英文關鍵字: --