- 作者: 王拯宗; 蕭裕源; 劉步遠; 侯書文; 關學婉
- 作者服務機構: 國立臺灣大學醫學院牙醫學系; 國立臺灣大學醫學院病理學科
- 中文摘要: 在有關顳顎關節的研究領域中,經由病理解剖取得的完整顳顎關節極具研究價值。但欲將此種兼含硬軟組織的大型標本製成組織切片時,以往所使用的方法既耗時費事,且又遭遇有關標本定位、脫鈣、切片等方面的困難。本篇報告針對上述問題,提出一個簡單可靠的製作方法。 此一方法的步驟重點如下:在脫鈣之前,以自凝樹脂將標本固定於夾具上,利用X光攝影進行標本定位並作必要調整以選擇所希望的切割方向,即可以慢速骨鋸將整個顳顎關節標本沿著選定方向連續切成4至5個厚度為3mm的薄片。所有薄片分別經肉眼及解剖顯微鏡觀察後再以現有最和緩脫鈣劑一EDTA進行脫鈣,並經例行石腦切片製作程序取得組織切片。 使用本方法處理顳顎關節標本,可在解剖後20至24天內完成組織切片製作。依照脫鈣前各薄片連續切割的順序,組合取自各薄片的組織切片,即可獲得一組問隔3mm跳切,具有代表性的組織切片,且鏡下觀察顯示各關節組成構造的相對位置維持良好,組織細胞的形態細節保存良好。 藉著此一標本製作方法,可在利用X光攝影所選定的參考平面上,經由肉眼及顯微鏡觀察進行顳顎關節的局部解剖學研究。不僅可詳細觀察每一個顳顎關節的組織細節,並可充分了解任何顳顎關節病變在各組成構造的對應位置上所引起的各種主要、次發病理變化。 本方法並可廣泛應用於身體其他部位,兼含硬軟組織標本的組織學、病理學研究。
- 英文摘要: Ten fresh temporomandibular joint (TMJ) specimens about 5 x 4.5 x 3.5 cm in size were removedat autopsy by 5 cuts according to appropriate anatomical landmarks. After routine formalin fixation, thewhole-TMJ specimens were wrapped with a thin layer of self-curing resin and then cut with a low speedbone saw along the parasagittal plane predetermined by x-ray guidance. Each specimen was serially cut into4 to 5 parallel slices of 3 mm thickness, which were then decalcified with 14% EDTA and embedded inparaffin. Histological sections of 5 microns were stained with hematoxylin and eosin. The procedures wereaccomplished within 20 to 24 days after autopsy. With this technique, the anatomical interrelationshipsamong the various joint components could be maintained and the macroscopic and microscopic topographyof the TMJ could be studied in the desired reference plane. Therefore, the corresponding changes amongthe joint components in a diseased TMJ could be thoroughly examined. This technique was also applicablefor the study of large specimens containing both hard and soft tissues.
- 中文關鍵字: whole-TMJ specimen; X-ray orientation; undecalcified slicing; EDTA decalcification
- 英文關鍵字: --