- 作者: --
- 作者服務機構: Department of Chemistry, Aligarh Muslim University, Aligarh-202 002, Uttar Pradesh, India
- 中文摘要: A kinetic spectrophotometric method has been described for the determination of metoprolol tartrate in commercial dosage forms. The procedure is based on the reaction of the drug with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethylsulfoxide (DMSO) at 100 ± 1 °C. The reaction is investigated by measuring the change in absorbance with time at 420 nm. Fixed-time (DA) and equilibrium methods are chosen for obtaining the calibration curves. Both calibration curves were found to be linear over the concentration range of 5-60 mg mL-1. The regression analysis of calibration data resulted in the linear regression equations of DA = -1.608 ’ 10-4 + 3.96 ’ 10-3 C and A = 7.31 ’ 10 -4 + 1.90 ’ 10-2 C for fixed time (DA) and equilibrium methods, respectively. The limit of detection (LOD) for fixed time and equilibrium methods are 1.16 and 0.415 mg mL-1, respectively. The method has been successfully applied to the quantitation of metoprolol tartrate in commercial dosage forms. Statistical comparison of the results shows that there is no significant difference between the proposed methods and El-Ries’s spectrophotometric method.
- 英文摘要: --
- 中文關鍵字: Metoprolol tartrate; 1-Chloro-2,4-dinitrobenzene; Meisenheimer complex; Pharmaceutical formulations; Spectrophotometry; Validation.
- 英文關鍵字: --