- 作者: Mei-Jie Jou a, Shuo-Bin Joub, Hung-Ming Chen c, Chi-Hung Lin d, Tsung-I Peng e
- 作者服務機構: a Department of Physiology and Pharmacology, Chang Gung University, Tao-Yuan, b Department of Neurology, China Medical College Hospital, Taichung , c Division of Emergency Medicine, Taipei Municipal Jen-Ai Hospital, d Institute of Microbiology and Immunology, National Yang Ming University, Taipei, e Department of Neurology , Lin-Kou Medical Center, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan, ROC
- 中文摘要: --
- 英文摘要: irradiated astrocytes. The antioxidants melatonin andvitamin E largely attenuated laser irradiation-inducedmROS formation and prevented apoptosis. Cyclosporin A(CsA), a mitochondrial permeability transition (MPT)blocker, did not prevent visible laser irradiation-inducedmROS formation and apoptosis. In conclusion, mROS for-mation contributes significantly to visible laser irradiation-induced apoptosis via an MPT-independent pathway.Laser irradiation-induced phototoxicity has been inten-sively applied in clinical photodynamic therapy for thetreatment of a variety of tumors. However, the preciselaser damage sites as well as the underlying mecha-nisms atthe subcellular level are unknown. Using a mito-chondrial fluorescent marker, MitoTracker Green, severemitochondrial swelling was noted in laser-irradiated ratbrain astrocytes. Nucleus condensation and fragmenta-tion revealed by propidium iodide nucleic acid stainingindicated that laser-irradiated cells died from apoptosis.Using an intrace llular reactive oxygen species (ROS) flu-orescent dye, 2',7'-dichlorofluorescin diacetate, hetero-geneous distribution of ROS inside astrocytes was ob-served after laser irradiation. The level of ROS in themitochondrial compartment was found to be higher thanin other parts of the cell. With another ROS fluorescentdye, dihydrorhodamine-123, and time-lapse laser scan-ning confocal microscopy, a substantial increase in mito-chondrial ROS (mROS) was visualized in visible laser-
- 中文關鍵字: --
- 英文關鍵字: Phototoxicity,· Apoptosis, Mitochondria reactive oxygen species, Laser scanning microscopy, Dihydrorhodamine-123