- 作者: Joel Gatlin; Salvatore J. Arrigo; Michael G. Schmidt
- 作者服務機構: Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, S.C., USA
- 中文摘要: --
- 英文摘要: The maturation of human immunodeficiency type-1 virions is accomplished through the proteolytic processing of Gag and GagPol precursor proteins by the viral protease (PR). Since virions must be assembled at the cell surface from uncleaved precursor molecules, intracellular activation of PR must be inhibited. We have previously developed a system where the intracellular activity of PR, associated with GagPol, was inhibited by the expression of Gag in trans. The disproportionate synthesis of Gag inhibits the activation of PR in the cytoplasm. Sequences capable of mediating this inhibition were localized to capsid. In this communication, the region of HIV-1 capsid capable of mediating inhibition was further defined and shown to require the major homology region of capsid within Gag.
- 中文關鍵字: --
- 英文關鍵字: HIV-1 protease; Regulation; Capsid; Major homology region