- 作者: Alireza Rezazadeh, Razieh Yazdanparast and Mahsa Molaei
- 作者服務機構: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
- 中文摘要: --
- 英文摘要:
Background: Nonalcoholic steatohepatitis (NASH), a progressive stage of nonalcoholic fatty
liver disease (NAFLD), is characterized by steatosis (accumulation of triacylglycerols within
hepatocytes) along with inflammation and ballooning degeneration. It has been suggested that
oxidative stress may play an important role in the progress of NAFLD to NASH. The aim of
present study was to determine whether antioxidant supplementations using EUK-8, EUK-134
and vitamin C could improve the biochemical and histological abnormalities associated with
diet-induced NASH in rats.
Methods: NASH was induced in male N-Mary rats by feeding a methionine - choline deficient
(MCD) diet. The rats were fed either normal chow or MCD diet for 10 weeks. After NASH
development, the MCD-fed rats were randomly divided into four groups of six: the NASH group
that received MCD diet, the EUK-8 group which was fed MCD diet plus EUK-8, the EUK-134
group which was fed MCD diet plus EUK-134 and the vitamin C group which received MCD
diet plus vitamin C. EUK-8, EUK-134 and vitamin C (30 mg/kg body weight/day) were
administered by gavage for eight weeks.
Results: Treatment of MCD-fed rats with salens reduced the sera aminotransferases, cholesterol,
low density lipoprotein contents, the extent of lipid peroxidation and protein carbonylation
whereas the HDL-C cholesterol levels were significantly increased. In addition, EUK-8 and
EUK-134 improved steatosis, ballooning degeneration and inflammation in liver of MCD-fed
rats.Conclusion: Antioxidant (EUK-8, EUK-134 and vitamin C) supplementation reduces NASHinduced
biochemical and histological abnormalities, pointing out that antioxidant strategy could
be beneficial in treatment of NASH.
- 中文關鍵字: --
- 英文關鍵字: Nonalcoholic fatty liver, superoxide dismutase mimetic, oxidative stress, Mn-salen complexes, methionine and choline deficient diet.