• 作者： 鄭建中 ; 呂燕凌
• 作者服務機構： 中央研究院化學研究所
• 中文摘要： Salen-type Ru(III) complexes are found to be capable of reacting with physiologically acceptable oxidants. The water solubility and DNA affinity of these Ru(III)-salen complexes are enhanced by the utilization of a variety of charged groups through the formation of peptide bonds. In the presence of hydrogen peroxide, modified Ru(III)-salen complexes are capable of nicking DNA. In addition, the reactivity in DNA cleavage increases along with the total number of positive charges retaining in Ru( III)-salen complexes and less influence in the electronic effect. Using /sup 32/P-end-labeled oligonucleotides and high resolution polyacrylamide gel electrophoresis, Ru(III)-salen complexes are found to randomly cleave DNA regardless of the DNA secondary conformation such as bulge, inter-loop, or double-stranded regions. The possible reactive species of Ru(III)-salen complexes in DNA cleavage is considered as the hydroxyl radical and high valent oxoruthenium (IV) species according to the UV titration, quenching studies, and reaction with varied oxidants.
• 英文摘要： --
• 中文關鍵字： Ruthenium; Salen; Dna Cleavage; Hairpin Type Triplex
• 英文關鍵字： 釕；沙林；去氧核糖核酸剝離；髮夾式三螺旋