- 作者: Chin-Hung Tsai; Tai-Long Pan; Ying-Shiung Lee; Yen-Kuang Tai; Tsan-Zon Liu
- 中文摘要: --
- 英文摘要: Previous reports have suggested that high-dose L-argi-nine could be used in diabetic patients as a prophylacticblocker for the initial glycation reaction of proteins bymethylglyoxal(MG), a reactive dicarbonyl compound ofglucose metabolism. Here, we present several lines ofevidence to substantiate that this prophylactic interven-tion may be inappropriate and should be used with care.First, we demonstrated that when various concentra-tions of L-arginine(2.0-8.0 mM) were added to a fixedconcentration of MG(1.56 gM) in a buffered lucigeninsolution,dose-dependent generation of superoxide an-ion(O-2)-mediated ultraweak chemiluminescence(uwCL)occurs. The suppression of uwCL generation by exoge-nously added superoxide dismutase further substan-tiated that the interaction between MG and L-argininegenerated 0-2. This phenomenon can also be demon-strated in a serum-based system. Furthermore, when afixed concentration of L-arginine (8.0 mM ) was addedexogenously to a group of sera obtained from either dia-betic patients(n=10)or their matched nondiabetic con-trols(n=10), a marked discrepancy in the generation ofO-2-mediated uwCL could be demonstrated(12,534 ±3,147 vs. 950± 350 counts; p<0.001).Taken together,this evidence demonstrates that the appropriateness ofusing high-dose L-arginine for prophylactic measures indiabetic patients may be questioned, because the inhibi-tion of the glycation reaction between MG and proteinsby high-dose L-arginine unexpectedly produces pletho-ric O-2as a by-product, which may subsequently aggra-vate the preexisting oxidative stress status of diabeticpatients.
- 中文關鍵字: --
- 英文關鍵字: Mathylglyoxal; glycaion reaction; Superoxide generation; Prophylactic blocker; Diabetes mellitus