- 作者: Li-Yi Sun, Cheng-Yoong Pang, Dian-Kun Li, Chia-Hsin Liao, Wei-Chao Huang, Chao-Chuan Wu, Yi-Yo Chou, Wei Wu Li, Shin-Yuan Chen, Hwan-Wun Liu, Yao-Jen Chang and Ching-Feng Cheng
- 作者服務機構: Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, No. 707, Sec. 3, Zhongyang Rd., Hualien City 970, Hualien, Taiwan, R.O.C
- 中文摘要: --
- 英文摘要:
Background: Antioxidants have been shown to enhance the proliferation of adipose-derived mesenchymal stem cells (ADMSCs) in vitro, although the detailed mechanism(s) and potential side effects are not fully understood.
In this study, human ADMSCs cultured in ImF-A medium supplemented with antioxidants (N-acetyl-l-cysteine and ascorbic acid-2-phosphate) and fibroblast growth factor 2 (FGF-2) were compared with ADMSCs cultured with FGF-2 alone (ImF) or with FGF-2 under 5% pO2 conditions (ImF-H).
Results: During log-phase growth, exposure to ImF-A resulted in a higher percentage of ADMSCs in the S phase of the cell cycle and a smaller percentage in G0/G1 phase. This resulted in a significantly reduced cell-doubling time and increased number of cells in the antioxidant-supplemented cultures compared with those supplemented with FGF-2 alone, an approximately 225% higher cell density after 7 days. Western blotting showed that the levels of the CDK inhibitors p21 and p27 decreased after ImF-A treatment, whereas CDK2, CDK4, and CDC2 levels clearly increased. In addition, ImF-A resulted in significant reduction in the expression of CD29, CD90, and CD105, whereas relative telomere length, osteogenesis, adipogenesis, and chondrogenesis were enhanced. The results were similar for ADMSCs treated with antioxidants and those under hypoxic conditions.
Conclusion: Antioxidant treatment promotes entry of ADMSCs into the S phase by suppressing cyclin-dependent kinase inhibitors and results in rapid cell proliferation similar to that observed under hypoxic conditions.
The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production. - 中文關鍵字: --
- 英文關鍵字: Adipose-derived mesenchymal stem cells, Antioxidant, Fibroblast growth factor-2, Hypoxia