第11卷‧第12期 - 3,
198412
, pp. 1553-1558
助孕素之固相酵素免疫分析法
- 作者:
章淑貞; 周成功; 金鏗年; 何橈通; 柴惠珍; 宋曉雲; 宣立人; 林仁壽
- 作者服務機構:
榮民總醫院內科部﹑醫學研究部; 三軍總醫院醫學研究部; 國立台灣大學畜牧系
- 中文摘要:
利用酵素免疫分析法來測定血清中助孕素,是以助孕素蕖草根過氧化?複合體
(progesterone-llα-OCO CONH-HRPO)來作標識,及利用抗體一固相
免疫吸附體(solid phase immunosorbent)來分離結合型(bound fraction)和
游離型(free fraction)。本法既精準又簡便。
從25位健康女性,在月經前後一週左右,抽取其血液各一次,以此方法測定血
清中助孕素之含量。其分析資料顯見與正常經期吻合。故本法極具應用價值。t
- 英文摘要:
A solid phase enzyme immunoassayfor determination of progesterone inhuman serum is described, using pro-gesterone-11α OCO CONHHRPOas the“marker”, immunosorbent con-jugated antiserum was used for se-paration of free and bound fractions.The assay is precise, very sensitive(l0pg) and convenient. The serum levels of progesteronewere measured by the enzyme immu-noassay in 25 normal wemen. The datafit with physiological concepts of themenstration, suggesting that the solidphase enzyme immunoassay is anaffective method of serum progesteronedetermination.
- 中文關鍵字:
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- 英文關鍵字:
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