- 作者: 甘魯生 ; Bauman, Rick ; Bhan, Purshotam 等
- 作者服務機構: 中央研究院化學研究所
- 中文摘要: A dideoxyribonucleotide, 2'-amino-2'-deoxyuridylyl- 3',5'-deoxyuridine, containing an unusual base(2'-amino-2'-deoxyuridine) that is resistant to nucleases was investigated by /sup 1/H NMR. The pK/sub a/ of the protonation of the amino group (5.8) was determined by profiles of chemical shifts of protons in the vicinity of amino group versus pH. However, protonation of the amino group has little effect on the conformation of the dimer, assumed to be B-form DNA. This conclusion is drawn from the chemical shift data and coupling constants of H/sub 1'/-H/sub 2'/. Thus, 2'-amino-2'-deoxyuridine can be used in antisense and anticode oligonucleotides.
- 英文摘要: --
- 中文關鍵字: Nmr; Aminodeoxyuridylyl Deoxyuridine; Chemical Shift; Pk/Sub A/; Coupling Constant; Amino Group
- 英文關鍵字: 核磁共振光譜法;胺基去氧尿單磷酸基去氧尿嘧啶核;化學位移;pK/sub a/值;耦合常數;胺基