- 作者: Yu-Yu Tsenga and Shan-Ho Choua,b
- 中文摘要: Exchangeable proton information is of critical importance in the NMR structural studies of DNA/RNA oligomers and/or their complexes with protein. However, no systematic NMR assignment strategy about these protons has been reported until now, although similar studies about non-exchangeable proton assignment have been well established in the past decade. We now report detailed NMR studies of a DNA duplex (d20-mer) containing the recognition site (5?/font>-ATGAC-3?/font>) of GCN4 basic zinc finger protein. All exchangeable and non-exchangeable protons except H5?/font>/H5?/font> protons could be assigned from a single H2O/NOESY spectrum at 25 蚓. The assignment strategy reported in this paper shall be useful to NMR studies of unusual DNA/RNA structures and their complexes with peptides/proteins.
- 英文摘要: --
- 中文關鍵字: --
- 英文關鍵字: --