• 作者： Hasan ACAR; Munis DUNDAR; And Janet STEWART
• 作者服務機構： Department of Medical Genetics; Selcuk University Faculity of Medicine; Department of Medical Genetics; Erciyes University; Faculity of Medicine; Kayseri; Turkey; Department of Medical Genetics; Royal Hospital for Sick Children
• 中文摘要： 　　急性前白血球白血病(APL)具有特別之染色體變化：由染色體15q22移位到染色體17之q12，使得PML基因接合到染色體17上的RARα基因。本研究利用傳統之核型分析尋找染色體15至17之移位，再由雙色螢光原位雜交法在分裂間期(interphase)證實PML及RARα基因之接合。兩種方法得以在APL病人的系列骨髓或周邊血球檢體內發現病變細胞，結果發現第二種分子雜交技術的確有助於傳統染色體分析。
• 英文摘要： Acute promyelocytic leukemia (APL) is a malignant condition characterized by t(15;17)(q22;q12),which fuses the PML gene on chromosome 15 to the retinoic acid receptor alpha (RAR ) gene on chromo-some 17. In this study, t(15;17) was identified cytogenetically by using the conventional cytogenetic tech-nique, and its molecular counterpart RAR /PML fusion on chromosome 17 on interphase nuclei was furtherconfirmed by means of dual color-（DC-）fluorescence in situ hybridization (FISH）on serial bone marrow(BM) and peripheral blood (PB) samples from APL patients at different stages of the disease. Overall, ourfindings indicate that interphase DC-FISH analysis can be a useful technique as an adjunct to conventionalcytogenetic investigation for detecting the presence of RAR /PML fusion in APL.
• 中文關鍵字： APL;t(15;17)(q22;q12-21); RARα/PML; fluorescence in situ hybridization.
• 英文關鍵字： --