- 作者: Hsu-Chen Chiu; Wei-Hao Liao; Szu-Wwe Chen; Chin-Tien Wang
- 作者服務機構: Institute of Clinical Medicine, National Yang-Ming University School Medicine and Department of Medical Research and Eeucation, Taipei Veterans General Hhspital, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: To elucidate the role of the C-terminal portion of Gag inthe incorporation of human immunodeficiency virustype 1 (HIV-1) Gag-Pol into virus particles, a series ofHIV-1 Gag-Pol mutants with deletions in the C-terminalgagsequence was constructed and viral incorporation ofthe Gag-Pol deletion mutants was analyzed using co-transfecting 293T cells with a Pr55 expression plas-mid. The biological function of the incorporated HIV-1polgene product was tested using an infectivity assay ofthe released virus particles which were pseudotypedwith the murine leukemia virus Env. Analysis indicatedthat Gag-Pol deletion mutants, with a removal of thematrix (MA) and/or nucleocapsid (NC) or of the N-termi-nal two thirds of the gag coding sequence, could beincorporated efficiently into virus particles and producesignificant amounts of infectious virions when assayedin a single-cycle infection assay. In contrast, mutationsinvolving a deletion of the major homology region andthe adjacent C-terminal capsid sequence significantlyaffected Gag-Pol incorporation. However, incorporationinto virus particles of a Gag-Pol deletion mutant retain-ing both the major homology region and the adjacentC-terminal capsid intact was still severely impaired. Thissuggests that the capsid major homology region and theadjacent C-terminal capsid sequence in Gag-Pol are nec-essary but not sufficient for the incorporation of HIV-1Pr1609a9-P0i into virus particles.
- 中文關鍵字: --
- 英文關鍵字: --