- 作者: 林淵泉
- 作者服務機構: Department of Chemistry, Biological Chemistry, and the Molecular Biology Institute, University of California, Los Angeles, California 90024, USA
- 中文摘要: In earlier work we have described and partially characterized two histone kinases from regenerating rat liver and other tissues which catalyze the transfer of the γ-phosphoryl group from ATP to histones. The histone phosphates thus formed were observed to be acid-labile and base-stable. In the present study we report on the specificity of one of one of these enzymes, namely, the kinase which is optimally active at pH 9. this enzyme appears to be relatively specific for the two histidine residues of histone H4. these histidines occur at positions 18 and 75, and both are phosphorylated. However, when regenerating rat liver was the source of enzyme, the product was 1-phosphohistidine, whereas the enzyme from Walker-256 carcinosarcoma catalyzed the formation of 3-phosphohistidine.
- 英文摘要: --
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