• 作者： 蕭明熙; 施美玲
• 作者服務機構： 榮民總醫院醫學研究部
• 中文摘要： 生長調節素是植物自身調控生長與分化的重要因子，並且和生長期間啟動特異基因表現的關係非常密切。開發溴乙
  基荼酮，俾一併將離層酸，徒長素和??乙酸等三型植物生長調節素依其共通的羧酸官能基作成強紫外光吸收的衍生物
  ，配合逆相式高效液相層析法，首次完成二期水稻（臺農67）幼苗中及開花至熟成期間的種子內三種生長調節素的相互變
  化分析。得知經2μM GA 處理的二週齡幼苗，其??乙酸含量增加52％，同期的離層酸含量則低至不可定量檢測。配
  合膠體電泳法分析未熟成水稻種子內的貯存蛋白亦得知：貯存蛋白在開花後4～10天內開始大量表現，此階段正緊隨於水
  稻徒長素（GA ）和離層酸互為最大消長之時機，貯存蛋白約在水稻開花30天後即不再明顯增加。
  陽
• 英文摘要： Plant growth regulators are endogenous factors governing the dynamic processes of plant develop-ment. They could trigger specific gene expression at particular growth stages. α-Bromo-2'-acetonaphthonewas here established as a derivatization agent for the common carboxyl functional groups among abscisicacid, gibberellins, and indole-3-acetic acid. Derivatization, combined with reversed phase high performanceliquid chromatography, enabled simultaneous determination of multiple PGRs in seedlings and developingseeds of Oryza sativa cv. Tainung 67. Treatment of 2 μM GA was found to increase IAA level in 14-day-old seedlings by 52%. Mean-while, the ABA content was so extremely low that quantitative analysis was not feasible. An attempt wasalso made to correlate storage protein formation with changes of multiple PGRs in immature seeds. Resultsshowed that a majority of polypeptides of storage proteins appeared mainly between 4 and 10 DAF. Thiscorresponded to the timing that closely followed a drastic change between GA and ABA in the oppositedirection. Accumulation of storage proteins in developing seeds ceased at about 30 DAF.
• 中文關鍵字： plant growth regulators; storage proteins; HPLC; Oryza sativa
• 英文關鍵字： --