- 作者: Chien-Ying Liu; Peter I.Chuang; Chun-Liang Chou; Shu-Min Lin; Hao-Cheng Chen; Paichien Chou; Yun-Hen Liu; Chih-Ten Yu; Chun-Hua Wang; Horng-Chyuan Lin; Han-Pin Kuo
- 作者服務機構: Departments of Thoracic Medicine II and Thoracic Surgery, Chang-Gung Memorial Hospital, Taipei, Taiwan; Department of Medicine, University of Washington, Seattle, Wash., USA
- 中文摘要: --
- 英文摘要: The ability to generate reactive oxidative intermediatesis one of the quintessential properties of mature humanneutrophils. Endogenously generated oxidants havebeen shown to be an important mechanism underlyingneutrophil cell death. In acute lung inflammation, newlyrecruited neutrophils further encounter external oxi-dants, including reactive oxygen and nitrogen interme-diates. In our present study, we showed that A1,a consti-tutive and inducible Bcl-2 homologue expressed in ma-ture circulating human neutrophils, might conferthe pro-tection from hydrogen peroxide(H202)- and peroxyni-trite(ONOO)-induced cell death.Utilizing the myeloidprecursor cell line, HL-60, we further examined the hy-pothesis that A1 was capable of conferring cytoprotec-tive activity against these oxidative stresses. Whereasthe control-transfected HL-60 cells expressed smallamounts of A1 and were sensitive to the biologically rel-evant, cell death-inducing oxidants, H202 and ONOO, thestable transfectants that overexpressed A1 were signifi-cantly more tolerant. Furthermore, there was a correla-tion between the level of A1 expression and the anti-apoptotic activity. Thus, our results suggest a cytopro-tective role of A1 in mature human neutrophils underoxidant stresses in host defense and inflammation.
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- 英文關鍵字: --