- 作者: Hugo Vankelecom Eve Seuntjens Annelies Hauspie Carl Denef
- 作者服務機構: Department of Molecular Cell Biology, Laboratory of Cell Pharmacology, University of Leuven (K.U. Leuven), Medical School, Campus Gasthuisberg, Leuven, Belgium
- 中文摘要: --
- 英文摘要: We previously reported that transgenic ablation of go-nadotrophs results in impaired development of cellsimmunostainable for prolactin(PRL) but not of cellsimmunostainable for growth hormone(GH)or pro-opiomelanocortin( POMC)in pituitary of newborn mice.The question remained whether this reduction in PRLprotein is a reflection of reduced PRL mRNA expression,or whether this regulation is only situated at the transla-tional level.We therefore generated a new series oftransgenic mice in which gonadotrophs were ablated bydiphtheria toxin A targeting,and analyzed hormonemRNA levels instead of hormone protein around the dayof birth.Pituitary mRNA expression levels of luteinizinghormone-β(LHβ), PRL and GH were quantified usingreal-time TaqMan RT-PCR.Of the 13 transgenic miceobtained,8 showed a clear-cut reduction(ranging from62 to 98%)in LHβ mRNA levels. PRL mRNA values weresignificantly reduced in the transgenic mice(p=0.0034),while GH mRNA expression was unaffected(p=0.93).An additional observation was that female newbornmice produce 5 times more LHβ mRNA than male micewhereas no sex difference was observed for expressionlevels of PRL and GH mRNA. Moreover, in the wild-typemice, LHβ mRNA expression was 20-fold higher than GHmRNA expression which in turn was 500- to 1,000-foldhigher than PRL mRNA expression,suggesting a lowexpression level of the PRL gene at birth.In conclusion,the present data support the hypothesis that embryonicdevelopment of PRL gene expression is stimulated bygonadotrophs.
- 中文關鍵字: --
- 英文關鍵字: Transgenic mouse. Targeted cell ablation. Diphtheria toxin A. Pituitary. Messenger RNA. Quantitative RT-PCR. Gonadotroph. Prolactin. Growth hormone. Luteinizing hormone