• 作者： Huang, Fang-Cheng; Ju, Yi-Hsu
• 中文摘要： 本研究將產自Rhizopus arrhizus的脂解酵素以物理吸附的方式,將之固定於具有微孔的聚丙烯擔體上,以此固定化酵素,在正己烷及低水量下,做棕櫚油分餾物與硬脂酸的酯交換反應,期能獲得可可奶油替代品。本研究探討各種反應參數對此反應的影響,包括水含量、酵素量、溫度,基質濃度及硬脂酸與棕櫚油分餾物莫耳比的影響。另外,對固定化酵素的儲存與操作穩定性亦予以試驗。結果顯示,酵素置於 4.degree.C冰箱68天後,其酯交換活性並沒有下降,而操作的半衰期近乎7天。
• 英文摘要： Lipase from Rhizopus arrhizus was immobilized by physical adsorption on hydrophobic microporous polypropylene supports with a specific activity of 0.15U/mg-support. The immobilized enzyme catalyst was employed for the interesterification of palm midfraction and stearic acid in the presence of n-hexane and low water content. The effects of reaction parameters which include water content, enzyme loading, temperature, the molar ratio of stearic acid to palm midfraction and substrate concentration were examined. Immobilized lipase retains nearly the same sigma value as that of fresh immobilized lipase after 68 days of storage at 4.degree.C. The half-life of the immobilized lipase with 0.2% w/v water content is ca. 7 days.
• 中文關鍵字： 物理吸附; 聚丙烯; 酵素; 催化劑; 固定化
• 英文關鍵字： Physical Adsorption; Polypropylene; Enzyme; Catalyst; Immobilize