- 作者: 陳世晞
- 作者服務機構: 中華醫專食品營養科
- 中文摘要: The enantioresolution of thirteen methylthiohydantoin- amino acids (MTH-amino acids), which are substrates for producing D-amino acids through enzyme-catalyzed hydrolysis, is described on R,S-2-hydroxypropyl derivatized .beta.- cyclodextrin bonded stationary phase (RSP-.beta.-CD CSP) with a water-based mobile phase. The enantioresolution is relatively sensitive to the structural variation of group which is attached to the carbon at position five on methylthiohydantoin moiety and subsequently turns into side- chain group of corresponding D-amino acid produced after hydrolysis. The inclusion complexation is believed to be the mechanism responsible for the observed enantioresolution that cannot be reproduced either on native .beta.- cyclodextrin CSP under the same chromatographic conditions or on both CSPs using the acetonitrile-based mobile phase. Approaches for enantiomers improvement include varying the percentage of organic modifier in the mobile phase and using the buffer- typed mobile phase such as triethylammonium acetate (pH 4.1).
- 英文摘要: --
- 中文關鍵字: Enantioresolution; Methylthiohydantoin; Amino Acid; Water- Based Mobile Phase; Stationary Phase; Cyclodextrin
- 英文關鍵字: 對映解析度;甲基吩內醯;胺基酸;水基移動相;靜相;環糊精