- 作者: Aliakbar Rajabzadeh, Alireza Ebrahimzadeh Bideskan, Alireza Fazel, Mojtaba Sankian, Houshang Rafatpanah and Hossein Haghir
- 作者服務機構: Department of Anatomy and Cell Biology, Mashhad, Iran
- 中文摘要: --
- 英文摘要:
Background: Maternal epileptic seizures during pregnancy can affect the hippocampal neurons in the
offspring. The polysialylated neural cell adhesion molecule (PSA-NCAM), which is
expressed in the developing central nervous system, may play important roles in neuronal
migration, synaptogenesis, and axonal outgrowth. This study was designed to assess the
effects of kindling either with or without maternal seizures on hippocampal PSA-NCAM
expression in rat offspring.
Methods: Forty timed-pregnant Wistar rats were divided into four groups: A) Kind+/Seiz+, pregnant
kindled (induced two weeks prior to pregnancy) rats that received repeated intraperitoneal
(i.p.) pentylenetetrazol, PTZ injections on gestational days (GD) 14-19; B) Kind-/Seiz+,
pregnant non-kindled rats that received PTZ injections on GD14-GD19; C) Kind+/Seiz-,
pregnant kindled rats that did not receive any PTZ injections; and D) Kind-/Seiz-, the sham
controls. Following birth, the pups were sacrificed on PD1 and PD14, and PSA-NCAM
expression and localization in neonates’ hippocampi were analyzed by Western blots and
immunohistochemistry.
Results: Our data show a significant down regulation of hippocampal PSA-NCAM expression in the
offspring of Kind+/Seiz+ (p = 0.001) and Kind-/Seiz+ (p = 0.001) groups compared to the
sham control group. The PSA-NCAM immunoreactivity was markedly decreased in all parts
of the hippocampus, especially in the CA3 region, in Kind+/Seiz+ (p = 0.007) and Kind-/Seiz+
(p = 0.007) group’s newborns on both PD1 and 14.
Conclusion: Our findings demonstrate that maternal seizures but not kindling influence the expression of
PSA-NCAM in the offspring’s hippocampi, which may be considered as a factor for
learning/memory and cognitive impairments reported in children born to epileptic mothers. - 中文關鍵字: --
- 英文關鍵字: Maternal Seizure, Polysialylated Neural Cell Adhesion Molecule, Kindling, Rat Hippocampus