- 作者: Ching-iang Huang, Mei-Chiao Wu
- 作者服務機構: Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, National Taiwan University, Taipei Taiwan, ROC
- 中文摘要: --
- 英文摘要: by the WEs of the 'cooling foods', chrysanthemum flow-er, bitter gourd,and lotus seed plumule, with respectiveIC s of 0.6,0.13,and0.08 mg/ml. At the concentrationstested, none of the EAEs had any effect on basal PGEproduction, while LPS-induced PGE production was in-hibited or increased by the EAE from bitter gourd andlongan, respectively. Water-soluble extracts of foods tra-ditionally regarded as 'heating' enhanced basal PGEproduction, while those from 'cooling' foods significant-1y inhibited LPS-induced PGE production by the macro-phage cell line. This subject merits further study to deter-mine whether appropriate food selection may help pa-tients suffering from chronic inflammatory conditions.Some components of natural foods may enhance orinhibit prostaglandin formation and potentially affectthe inflammation condition. A macrophage cell line,RAW264.7, was employed to examine the effects offoods traditionally regarded as 'heating' or 'cooling' onthe production of PGE , a well-known proinflammatorymediator. Foods traditionally regarded as 'heating' (lit-chi, longan, and dried longan) or 'cooling '(chrysanthe-mum flower, bitter gourd, and lotus seed plumule) wereextracted sequentially with water and ethyl acetate. Thewater extracts (WE) and ethyl acetate extracts (EAE) wereapplied to RAW264.7 macrophages in the presence orabsence of LPS (lipopolysaccharide). In the absence ofLPS, the WEs from the 'heating foods', litchi, longan, ordried longan had a dose-dependent enhancing effect onPGE production, with respective EC s of 8.4, 16, and11 mg/ml. This effect was accompanied by significantinduction of COX-2 protein expression,as shown byWestern blot analysis. In contrast, LPS-induced PGEproduction was inhibited in a dose-dependent manner
- 中文關鍵字: --
- 英文關鍵字: COX-2, Food, Macrophage, PGE2, Prostaglandin production, Traditional food belief