• 作者： Wu, C.B.; Kuo, S.M.; Chang, S.J.et al.
• 中文摘要： 本研究藉由苯甲酸鈉及苯甲酸在UV光照射下,進行交聯反應以製備聚乙烯醇(PVA)薄膜。再利用三聚氯化氰將盤尼西林酵素固定在PVA薄膜上,並結合pH玻璃電極,以製成盤尼西林感測器。經由實驗結果得知,氫離子在此薄膜的擴散速率高出盤尼西林-血清白蛋白薄膜100倍,其擴散係數為1.4*10/sup -6/cm/sup 2//sec。增加緩衝液的離子強度會使氫離子穿透速率變快,卻降低酵素電極的靈敏度。但高強度的緩衝液可擴大盤尼西林電極的線性分析範圍。由結果證實,聚乙烯醇水膠可作為感測器之良好酵素固定基材。
• 英文摘要： Poly(vinyl alcohol), PVA, membrane was prepared by crosslinking reaction initiated by UV irradiation in the presence of sodium benzoate and benzoic acid. Penicillinase was immobilized after the PVA membrane had been activated with cyanuric chloride. The penicillinase-immobilized PVA membrane was then coupled with a pH glass electrode and used as a penicillin sensor. The effective diffusion coefficient of hydrogen ions through the penicillinase-immobilized PVA membrane was determined to be about 1.4*10/sup -6/cm/sup 2//sec. This value is about two orders of magnitude higher than that obtained from the penicillinase-BSA (bovine serum albumin) membrane. Increasing the buffer strength increased the mass transfer rate of hydrogen ions, but decreased the sensitivity of the enzyme electrode. The higher buffer strength nevertheless increased the linear analytical range of the penicillin electrode. These results demonstrate the applicability of using PVA hydrogel as an excellent enzyme immobilized matrix for biosensor.
• 中文關鍵字： 聚乙烯醇; 膜; 交聯; 盤尼西林; 電極; 紫外光
• 英文關鍵字： Polyvinyl Alcohol; Membrane; Crosslink; Penicillin; Electrode; Ultra Violet