- 作者: Adriana Degrossoli; Marcelle Carolina Colhone; Wagner Welber Arrais-Silva; Selma Giorgio
- 作者服務機構: Depatrment of Parasitology, Biology Institue, Universidade Estadual de Campinas, Campinas, Brazil
- 中文摘要: --
- 英文摘要: Hypoxia,a microenvironmental factor present in dis-eased tissues, has been recognized as a specific meta-bolic stimulus or a signal of cellular response.Experi-mental hypoxia has been reported to induce adaptationin macrophages such as differential migration,elevationof proinflammatory cytokines and glycolytic enzyme ac-tivities, and decreased phagocytosis of inert particles. Inthis study we demonstrate that although exposure tohypoxia (5% 02, 5% C02, and balanced N2) did notchange macrophage viability, or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) cleavageand proliferation,it significantly reduced expression ofthe 70-kD heat shock protein (HSP70),which was re-stored to prehypoxia levels after reoxygenation.Theinfluence of low oxygen tension on macrophage func-tional activity was also studied,i.e. the ability of thesecells to maintain or resist infection by a microorganism.We demonstrate that macrophages from two differentsources (a murine cell line and primary cells) exposed tohypoxia were efficiently infected with Leishmania ama-zonensis, but after 24 h showed a reduction in the per-centage of infected cells and of the number of intracellu-lar parasites per macrophage, indicating that hypoxiainduced macrophages to kill the intracellular parasites.These results support the notion that hypoxia, a mi-croenvironmental factor, can modulate macrophage pro-tein expression and functional activity.
- 中文關鍵字: --
- 英文關鍵字: --