- 作者: Youssef Sari, Zaneer M Segu, Ahmed YoussefAgha, Jonathan A Karty and Dragan Isailovic
- 作者服務機構: 1Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, U.S.A.
- 中文摘要: --
- 英文摘要:
Background: A derived peptide from activity-dependent neurotrophic factor (ADNF-9) has been shown
to be neuroprotective in the fetal alcohol exposure model. We investigated the neuroprotective effects of
ADNF-9 against alcohol-induced apoptosis using TUNEL staining. We further characterize in this study
the proteomic architecture underlying the role of ADNF-9 against ethanol teratogenesis during early fetal
brain development using liquid chromatography in conjunction with tandem mass spectrometry (LCMS/
MS). Methods: Pregnant C57BL/6 mice were exposed from embryonic days 7-13 (E7-E13) to a
25% ethanol-derived calorie [25% EDC, Alcohol (ALC)] diet, a 25% EDC diet simultaneously
administered i.p. ADNF-9 (ALC/ADNF-9), or a pair-fed (PF) liquid diet. At E13, fetal brains were
collected from 5 dams from each group, weighed, and frozen for LC-MS/MS procedure. Results:
Administration of ADNF-9 prevented alcohol-induced reduction in fetal brain weight and alcoholinduced
increases in cell death. Moreover, individual fetal brains were analyzed by LC-MS/MS.
Statistical differences in the amounts of proteins between the ALC and ALC/ADNF-9 groups resulted in
a distinct data-clustering. Significant upregulation of several important proteins involved in brain
development were found in the ALC/ADNF-9 group as compared to the ALC group. Conclusion: These
findings provide information on potential mechanisms underlying the neuroprotective effects of ADNF-9
in the fetal alcohol exposure model. - 中文關鍵字: --
- 英文關鍵字: --