- 作者: 紀朗明 ; 謝昌煥 ; 吳文桂
- 作者服務機構: 清華大學生命科學研究所
- 中文摘要: Solid-state high resolution /sup 13/C NMR spectra of bovine brain sulfatide, egg sphingomyelin, egg phosphatidylcholine, and cholesterol/phosphatidylcholine dispersions were obtained by the cross-polarization/magic angle spinning (CP/MAS) method as functions of contact time, temperature and composition. Quantitative analysis of the chemical shift and linewidth data indicate that in the liquid-crystalline state the hydrocarbon chains of sulfatide are packed less orderly than those of sphingomyelin, and that there are two or more conformers for the ceramide residues of natural sphingolipids. The fatty acid double bond showed higher mobility than that of sphingosine as monitored by the signal intensity profiles as a function of contact time. The same approach was used to study the coexisting L/sub .alpha./ (liquid-crystalline phase) and.beta. (cholesterol-rich characterized phase) two-phase region of cholesterol/phosphatidylcholine mixtures. Without isotope enrichment, structural and phase properties of natural lipid dispersions can be obtained easily by monitoring the chemical shift, the signal intensity and the linewidth of /sup 13/C NMR spectra.
- 英文摘要: --
- 中文關鍵字: Cross Polarization; Double Bond; Lipid; Dispersion; Cholesterol; Carbon 13 Nuclear Magnetic Resonance; Carbon 13 Nmr
- 英文關鍵字: 正交偏振;雙鍵;脂質;分散;膽固醇;碳13核磁共振