- 作者： 曾浩洋; Bruce A. Roe
- 作者服務機構： 國立中學大學食品科學系; 美國俄亥俄州肯特大學化學系
- 中文摘要：
為研究哺乳類之苯胺酸轉移核醣核酸（tRNAphe〉所含核醣胸腺核?（ribothymidine）含量對聚尿核?（poly U）
指導之聚苯胺酸（polyphe）生合成的影響；各種哺乳類苯胺酸轉移核醣核酸（具相同核?程序排列，僅由3'一端點算起
第23個位置之核醣胸腺?普含量不同）被用來做氨基酸化（aminoacylation）及轉移核醣核酸－核醣體（tRNA-ribosome
）結合反應之動力學實驗。這些苯胺酸轉移核醣核酸有由人類胎盤分離者，具50％之核醣胸腺核?含量；由牛肝分離者，
具72％之核醣胸腺核?，以及完全甲基化之牛肝苯胺酸轉移核醣核酸，具95％之核醣胸腺核?含量。研究結果顯示哺乳類
之苯胺酸轉移核醣核酸之核醣胸腺核?含量，既不影響其氨基酸化反應速率，也不影響苯胺酸轉移核醣核酸與核醣體結合
之速率；雖然以前的報告曾顯示核醣胸腺核?可影響聚尿核?指導之聚苯胺酸的生合成速率。l - 英文摘要： In order to study the mechanism for the effect of ribothymidine content of a specific mammaliantRNAPhe on the rate of poly(U) directed poly(Phe) synthesis, various mammalian tRNAPhe's differingonly in the ribothymidine content at position 23 from the 3'-terminus were employed for kinetic studiesfor both aminoacylation and Phe-tRNAPhe ribosome binding reactions. These mammalian tRNAPhe'semployed in study were human placenta tRNAPhe with 50% ribothymidine content，beef liver tRNAPhewith 72% ribothymidine content and completely methylated beef liver tRNAPhe with 95% ribothymidinecontent. Results showed that the ribothymidine content of specific mammalian tRNAPhe's differing onlyin the ribothymidine content affected neither the rate of aminoacylation nor the rate of Phe-tRNAPheribosome binding although it might affect the rate of poly(U) directed poly(Phe) synthesis.
- 中文關鍵字： --
- 英文關鍵字： --
