- 作者: Hung-Chuan Pan; Dar-Yu Yang; Shu-Peng Ho; Meei-Ling Sheu; Chung-Jung Chen; Shiaw-Min Hwang; Ming-Hong Chang; Fu-Chou Cheng
- 作者服務機構: Department of Neurosurgery, Taichung Veterans General Hospital, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Attenuation of inflammatory cell deposits and associated cytokines prevented the
apoptosis of transplanted stem cells in a sciatic nerve crush injury model. Suppression
of inflammatory cytokines by fermented soybean extracts (Natto) was also beneficial
to nerve regeneration. In this study, the effect of Natto on transplanted human
amniotic fluid mesenchymal stem cells (AFS) was evaluated. Peripheral nerve injury
was induced in SD rats by crushing a sciatic nerve using a vessel clamp. Animals
were categorized into four groups: Group I: no treatment; Group II: fed with Natto (16
mg/day for 7 consecutive days); Group III: AFS embedded in fibrin glue; Group IV:
Combination of group II and III therapy. Transplanted AFS and Schwann cell
apoptosis, inflammatory cell deposits and associated cytokines, motor function, and
nerve regeneration were evaluated 7 or 28 days after injury. The deterioration of
neurological function was attenuated by AFS, Natto, or the combined therapy. The
combined therapy caused the most significantly beneficial effects. Administration of
Natto suppressed the inflammatory responses and correlated with decreased AFS and
Schwann cell apoptosis. The decreased AFS apoptosis was in line with neurological
improvement such as expression of early regeneration marker of neurofilament and
late markers of S-100 and decreased vacuole formation. Administration of either AFS,
or Natto, or combined therapy augmented the nerve regeneration. In conclusion,
administration of Natto may rescue the AFS and Schwann cells from apoptosis by
suppressing the macrophage deposits, associated inflammatory cytokines, and fibrin
deposits. - 中文關鍵字: --
- 英文關鍵字: --