- 作者: --
- 作者服務機構: Department of Biochemistry & Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh
- 中文摘要: Three galactose-specific lectins have been isolated and purified from the extract of pumpkin seed kernels by gel filtration on Sephadex G-75 with 100% ammonium sulfate saturated crude extract, followed by ion exchange chromatography on DEAE-cellulose and affinity chromatography on Sepharose 4B. All three lectins were found to be homogeneous as judged by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weights of lectins, PSL-1, PSL-2 and PSL-3, as estimated by gel filtration on Sephadex G-75 were 40,000, 42,000 and 46,000, and by SDS-PAGE about 39,500, 41,000 and 45,000, respectively. The lectins, PSL-1, PSL-2 & PSL-3 were dimer in nature and the molecular weights of their subunits were about 25,500 and 14,000; 26,000 and 15,000; and 30,500 and 15,000, respectively. The lectins are glycoproteins with a neutral sugar content of 3-5%. The lectins agglutinated rat red blood cells and the hemagglutination was inhibited specifically by galactose and galactose-containing saccharides. The lectins exhibited a strong cytotoxic effect in a brine shrimp lethality bioassay
- 英文摘要: --
- 中文關鍵字: Pumpkin seed lectins; Purification; Galactose specific; Hemagglutination; Cytotoxicity; Sub-unit structure.
- 英文關鍵字: --