• 作者： 陳淑華; 鄭文玲; 許文輝
• 作者服務機構： 食品工業發展研究所; 菌種保存及研究中心; 私立嘉南藥學專科學校; 臺灣糖業研究所
• 中文摘要： 本實驗利用已建立之 Pseudomonas amyloderamosa isoamylase 基因（ISO）選殖系統，已從 isoamylase 高產突變株P. amyloderamosa JD210 選殖出帶有 ISO 基因之 DNA 片段。經次選殖分析，ISO基因包含於3.7kb DNA 片段內。ISO基因產物98%可分泌至胞外並可於 Pseudomonas aeruginosa, Pseudomonas putida 及 Escherichia coli 內表現；經isoamylase 活性分析與結合酵素免疫分析法證實其產物為 isoamylase。P. amyloderamosa ISO 轉形菌株之 isoamylase生產能力可於培養二天內達到高峰。以不同的碳源來培養ISO轉形菌株，發現以葡萄糖，麥芽糖及糊精為碳源時，轉形菌株及高產突變株均能生產 isoamylase。
• 英文摘要： The isoamylase gene (ISO) of isoamylase hyperproducing strain Pseudomonas amyloderamosa JD210was cloned in plasmid pKT230. Deletion analysis of the recombinant plasmid showed that the ISO codingsequence was located within a 3.7 kilobases DNA fragment. The ISO gene could be expressed inPseudomonas aeruginosa, Pseudomonas putida and Escherichia coli cells and their gene productswere identified as isoamylase by enzyme assay and immunoassay. ISO gene recombinant of P.amyloderamosa secreted most of the isoamylase into the growth medium and reached its maximal isoamy-lase productivity within two days. Carbon source studies of the ISO gene transformant and P. amylodera-mosa JD210 indicated that the ISO gene could be expressed in the medium containing glucose, maltoseor dextrin as a carbon source.
• 中文關鍵字： Pseudomonas anyloderamosa; isoamylase gene; gene expression
• 英文關鍵字： --