- 作者： 何純郎; 柯娟玲; 李鎮源
- 作者服務機構： 中央研究院生物學研究所; 國立臺灣大學醫學院藥理學研究所
- 中文摘要：
比較五種具強毒性之蛇毒磷脂?甲之藥理學作用，發現在小雞之頸神經一肌標本除了β-bungarotoxin外，所有被測
試之?在高濃度時均對acetylcholine之反應產生抑制，並使骨骼肌產生痙縮。幾乎所有由蛇毒分離得到之磷脂?甲均對
天竺鼠之紅血球產生間接溶血作用，少部份?甚至產生直接溶血作用，而β-bungarotoxin是唯一既不產生直接溶血亦不
產生間接溶血作用之?。所有被測試之?在極小濃度（0.05微克／毫升）便使天竺鼠之心房收縮力明顯增加，但β-bungaro-
toxin在?濃度高達10微克／毫升時，對此兩種肌標本仍然不產生刺激作用。對各種培養之細胞，β-bungarotoxin對神經
母細胞癌之細胞繁殖有抑制，但不直接殺死細胞；對大花鼠大腦之非神經細胞亦不產生細胞溶解作用。由以上各種結果
我們認為β-Bungarotoxin是目前所有已知之腱前神經毒素中最具選擇性的毒素。
. - 英文摘要： Five highly toxic phospholipases A (PLAs) (β-bungarotoxin, caudoxin, Mojave toxin, notexin anda basic PLA from Naja nigricollis venom) were compared for their pharmacological actions. In the chickbiventer cervicis nerve-muscle preparation, all PLA toxins except β-bungarotoxin (β-BuTX) inhibited thepostsynaptic acetylcholine response and induced contracture of the muscle at a high concentration. In-direct hemolytic activity was found in all PLA toxins and some of the toxins (Naja nigricollis basic PLAand Mojave toxin) even showed a potent direct hemolytic action, while β-BuTX was devoid of both directand indirect hemolytic activities on the guinea-pig erythrocytes. All PLA toxins except β-BUTX caused anincrease in muscle tone in the guinea-pig ileum at a concentration as low as 0.05 μg/ml, and an increase inthe contractile force in the guinea-pig atrium at a concentration of 1.0 μg/ml. In contrast, β-BuTX had nostimulant effect at concentrations up to 10 μg/ml. On the cultured cells, β-BuTX suppressed the prolife-ration of neuroblastoma cells, but did not cause lysis of non-neuronal cells of the rat brain. However, β-BuTX uniquely maintained a high population of viable cells in the neuroblastoma cell cultures. From theseresults it was concluded that β-BuTX is the most specific presynaptic neurotoxin among the PLA toxinsso far tested.
- 中文關鍵字： β-bungarotoxin; neurotoxic phospholipases A2; hemolysis; neuroblastoma cell; rat brain cell
- 英文關鍵字： --
