- 作者: Hironori Tsuchiya; Motohiko Nagayama
- 作者服務機構: Department of Dental Basic Education, Asahi University Schoolof Dentistry, Japan; Department of Oral Pathology, Asahi University School ofDentistry, Japan
- 中文摘要: --
- 英文摘要: As a novel approach to the mode of medicinalaction of garlic, its constituents were comparatively studiedwith respect to their interactions with membrane lipids tomodify the membrane fluidity. Allyl derivatives rigidifiedtumor cell and platelet model membranes consisting ofunsaturated phospholipids and cholesterol at 20–500 lMwith the potency being diallyl trisulfide (DATS)[diallyldisulfide (DADS) by preferentially acting on the hydrocarboncores of lipid bilayers. They were also effective inrigidifying candida cell model membranes prepared withergosterol and phospholipids at 100–500 lM with thepotency being DADS[DATS[diallyl sulfide (DAS), butnot bacteria cell model membranes without ergosterol.Alliin, a precursor of these DASs, was not active on anymembranes at 500 lM. Both relative intensity and selectivityin membrane effects correlated with those inantiproliferative, antiplatelet and antimicrobial effects. Incell culture experiments, membrane-active DASs inhibitedthe growth of tumor cells cultured for 24 and 48 h at 20–500 lM to show the potency being DATS [ DADS,together with rigidifying cell membranes by acting on theirdeeper regions more intensively. However, membraneinactiveallyl derivatives were not growth-inhibitory ontumor cells. The membrane lipid interactions of DASsappear to be one of possible mechanisms underlying differenteffects of garlic.
- 中文關鍵字: --
- 英文關鍵字: Allyl derivatives; Garlic; Membrane lipid interaction; Fluidity modification; Antiproliferative effect