• 作者： 徐奎望; 孟慶樑
• 作者服務機構： 國防醫學院牙醫學系及三軍總醫院牙科部
• 中文摘要： 本實驗使用四十隻金色倉鼠以研究口服大蒜對用 DMBA 在頰袋粘膜塗佈誘發癌症之抑制作用，將動物以隨機抽樣分為四個實驗組（分別先餵以大蒜，NTP,BP或礦物油，一週後在頰袋中塗佈DMBA以引發癌症）三個化學品對照組（分別餵以大蒜，NTP或BP，但不塗DMBA）和一個DMBA對照組（只在頰袋中塗DMBA）。自塗DMBA第四週起，每二週檢查動物之頰袋粘膜上腫瘤發生之情形（數目及大小）和血管之變化（數目及直徑），至塗DMBA後第二十六週犧牲全部動物，將頰袋及腫瘤全部取下，以檢查腫瘤之性質，粘膜細胞中DNA之合成量及GGTase之活動量。以此多種檢驗結果來評估大蒜，BP及NTP之抑癌效能，同時，動物之內臟亦取下，以檢測各試驗物之毒性。結果發現大蒜之抑癌效能顯然高於 BP 及 NTP 此種抑癌效能之機轉在癌發生之早期特為顯著，故或與大蒜中某些成份能與細胞核中的DNA結合，因而減低DMBA與DNA結合產生致癌作用有關。其他或與抑癌作用有關之因素包括局部血供不足，較低之GGTase活動和較少之DNA合成。故大蒜成份之萃取及每種萃取物之抑癌作用之測定將為在“大蒜之抑癌作用”一系列之實驗中合理而必需之一環。
• 英文摘要： The inhibitory effect of oral administration of garlic on experimental carcinogenesis in buccalpouches induced by painting 9,10-dimethyl-1,2-benz(a)anthracene (DMBA) was studied on 40 goldenSyrian hamsters. The animals were grouped at random into four experimental groups (oral administrationof garlic, NTP, BP or mineral oil followed by DMBA painting on buccal pouches), three chemical controlgroups (oral administration of garlic, NTP or BP without DMBA painting) and a DMBA control group (onlypainted DMBA on buccal pouches). Starting from the fourth week after DMBA painting, the pouch mu-cosae were examined biweekly for its tumor formation and blood vessel architecture. Animals were sacri-ficed 25 weeks after DMBA application. Tumors and pouch mucosae were dissected to examine tumornature and biochemical reactions of DNA synthesis and GGTase activity. The inhibitory efficacy of garlic,BP and NTP were evaluated according to the results of these examinations. Garlic was found to have ahigher inhibitory efficacy than BP and NTP through the probable mechanism of competitive binding withnuclear DNA and diminishing the opportunity of DMBA to initiate carcinogenesis. Other factors related tocancer inhibition included insufficient local blood flow, low GGTase activity and lesser DNA synthesis. Theinhibitory effect of fractions of garlic on experimental carcinogenesis should be a reasonable and necessarycontinuation in future studies of the series of cancer prevention by garlic.
• 中文關鍵字： inhibition; experimental carcinogenesis; garlic
• 英文關鍵字： --