- 作者: P.J.S. Chiu; G. Tetzloff
- 作者服務機構: Schering-Plough Research Institute, Kenilworth, N.J., USA
- 中文摘要: --
- 英文摘要: Endothelium-derived relaxing factor (EDRF) or nitric oxide (NO) biosynthesis from L-arginine occurs in the endothelium and platelets and may modulate plate- let function and contribute to thromboresistance in the vessel wall. A rat model was used to evaluate selective accumulation of ''In-labeled platelets in the pul- monary microcirculation following the administration of collagen, adenosine 5'- diphosphate (ADP) or thrombin. Platelet aggregation was monitored continu- ously over the thorax using a microcomputer-based system. Sodium nitroprus- side, a stimulator of soluble guanylate cyclase and zaprinast, a phosphodies- terase V inhibitor, both known to cause accumulation of cyclic guanosine mono- phosphate, exhibited moderate inhibitory activity, which was shared by L- arginine. NG-monomethyl-L-arginine (L-NMMA; 1 mg/kg/min), an inhibitor of EDRF(NO), potentiated the aggregatory response to collagen at an intravenous dose of 100 μg/kg but not at one of 30 μg/kg. D-NMMA had no such effect. The augmenting effect of L-NMMA was abolished by L-arginine. N0-nitro-L- arginine methyl ester (L-NAME; 0.1 mg/kg/min) also markedly augmented the collagen-induced platelet response, and, at higher doses, all treated animals died upon collagen challenge. Both L-NMMA and L-NAME did not affect the responses to ADP and thrombin. The results suggest that in the intact vascular system, basal release of EDRF(NO) is not critically involved in modulation of platelet function but becomes a significant factor when platelets are exposed to great amounts of collagen fibrils.
- 中文關鍵字: --
- 英文關鍵字: Endothelium-derived relaxing factor; Nitric oxide; Platelets; 'Indium oxine; Cyclic GMP; Zaprinast