• 作者： 何志?; 王聲遠; 歐柏榮
• 作者服務機構： 榮民總醫院醫學研究部
• 中文摘要： 用丙種干擾素(500 U/ml)短時(6小時)處理U937細胞，繼以正常培養液做長期（≧8週）培養，將可誘發出一種對干擾
  素之抗細胞及促分化效應具有抵抗性之細胞，其抗性可廣及濃度達之各型干擾素（丙種，甲種及乙種）。此外，抗
  性U937細胞尚變為對 phorbol 酯－－腫瘤提升劑(TPA)不敏感。不過，這些抗性細胞仍保持對不同類型干擾素抗病毒效
  應之敏感性，且亦能被干擾素誘導而產生2-5A合成?。抗性細胞群似呈均一性，因為由此細胞群中之單細胞所演發出來
  的同源細胞群(clones)皆呈現一致的對干擾素和TPA的抵抗表型。這些結果提示，抗性細胞之誘發，可能涉及一個去分
  化過程，它導致了對各型干擾素的促分化和／或抗細胞作用不起反應的幼稚細胞群的形成。e
• 英文摘要： A brief exposure (for 6 h) of U937 cells to interferon (IFN)-γ (500 U/ml) followed by a long termincubation of cells in normal medium for 8 or more weeks resulted in the induction of cells that wererefractory to the anticellular and differentiating effects of not only IFN-γ but also IFN-α and IFN-β atconcentrations up to U/ml. In addition, the cells became insensitive to the potent differentiating effectof the phorbol ester - tumor promoting agent (TPA). However, the resistant cells retained their sensitivityto the antiviral effect of different IFNs and were fullu responsive to the induction of endonuclease 2', 5'-oligoadenylate (2-5A) synthetase by IFN. Furthermore, the resistant cell population appeared to behomogeneous because clones derived from single cells from this population all exhibited the same resistantphenotype to IFN and TPA. These results suggest that induction of resistant U937 cells may involve adedifferentiation process which results in the formation of an immature cell population that do not respondto the differentiating and/or anticellular effects of various types of IFNs.
• 中文關鍵字： U937 cell; interferon resistance
• 英文關鍵字： --