- 作者: 陳水田 ; 李國芳 ; 王光燦
- 作者服務機構: Graduate Inst. Of Biochemical Science, National Taiwan Univ., R.O.C.
- 中文摘要: Procedures have been developed for the preparation of aspartyl containing dipeptides P-L-Asp-AA/sub 2/ (P=Boc, Cbz and Moz; AA/sub 2/=Phe-R or Ala-R; R=OH, OBzl and OTMB) using papain as a catalyst. The pH dependent of esterase activity by papain catalyzed hydrolysis have been studied. The papain catalyzing reaction can be reacted in aqueous solution, buffer solution or cosolvent system in a thermodynamically controlled approach. A procedure for the conversion of the artificial sweetener precursor P-Asp-Phe-OH to the aspartame is studied also.
- 英文摘要: --
- 中文關鍵字: Enzymatic Peptide Synthesis; Papain; Aspartame; Esterase Activity
- 英文關鍵字: 縮氨酸的合成;木瓜;阿斯巴甜;酯活性;胜的合成