- 作者: Jia-Hau Yen; Deng-Jye Yang; Meng-Chi Chen; Yu-Fan Hsieh; Yu-Shu Sun; Gregory J Tsay
- 作者服務機構: Institute of Immunology, Chung Shan Medical University, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background: To assess the effects of Glycine tomentella Hayata
(GTH), a traditional herbal medicine for treatment of rheumatic
diseases on the expression of the proinflammatory cytokines and
on the clearance of apoptotic cells by macrophages.
Methods: RAW264.7 cells were cultured with lipopolysaccharide
(LPS) in the presence or absence of ethanol extract of GTH. The
expression of proinflammatory cytokines IL-1β, IL-6, and TNF-α,
and inducible nitric oxide synthase (iNOS) and transglutaminase 2
(TG2) were assayed by reverse transcriptase-polymerase chain
reaction (RT-PCR) and enzyme-linked immunosorbent assay
(ELISA). Matrix metalloproteinase (MMP)-2 and MMP-9 were
assayed by gelatin zymography. For detecting uptake of apoptotic
cells, RAW264.7 cells were cultured with carboxyfluorescein
diacetate (CFDA)-stained apoptotic cells and assayed by flow
cytometry.
Results: The major components of GTH analyzed by
high-performance liquid chromatography (HPLC) chromatogram
were daidzein (42.5%), epicatechin (28.8%), and naringin (9.4%).
GTH treatment inhibited the expression of proinflammatory
cytokines IL-1β, IL-6 and MMP-9 but did not affect the expression of
TNF-α and iNOS. GTH significantly enhanced the expression of
TG2 and the clearance of apoptotic cells by RAW264.7 macrophages.
Conclusions: GTH inhibits proinflammatory cytokine secretion and
MMP-9 activity, enhances apoptotic cell uptake and up-regulates
TG2 expression. Our data show that GTH might have beneficial
effects on rheumatic diseases. - 中文關鍵字: --
- 英文關鍵字: --