- 作者: Frank Y.T. Tung; Vicky S. Kelley; James B. Hendricks
- 作者服務機構: a Department of Infectious Diseases and; Microbiology, Graduate School of Public; Health, University of Pittsburgh, Pa., and; b Department of Pathology and Laboratory; Medicine, College of Medicine, University of Florida, Gainesville, Fla., USA
- 中文摘要: --
- 英文摘要: Direct in situ introduction of retroviral producer cells might provide a form of treatment for localized tumors. A possible undesirable consequence of this treatment could be uncontrolled proliferation of the injected producer cells. To test this possibility, severe combined immunodeficiency (SCID) mice were reconstituted with human peripheral blood lymphocytes which were marked with a retroviral vector using a coculture method. Although specific measures were taken to remove the possible contaminating producer cells, a high per- centage of mice developed fibrosarcoma 2-6 weeks after reconstitution. We hypothesized that tumors arose from a small number of contaminating pro- ducer cells in the inoculum. Tumor cells were consistently DNA tetraploid, a characteristic of the producer cell line. DNA extracted from tumor tissue was found to contain the gene (neomycin phosphotransferase) used to mark the producer cell line. Furthermore, SCID mice injected with 1 x 10^4 producer cells developed tumors with analogous characteristics. This report indicates that the retroviral producer cell line is tumorigenic in immune-deficient ani- mals.
- 中文關鍵字: --
- 英文關鍵字: Retroviral vector; Genetherapy; SCID mice; Tumorigenesis