- 作者: Pei-Lan Shao; Po-Ren Hsueh; Luan-Yin Chang; Chun-Yi Lu; Chuan-Liang Kao; Yu-Ping Chiang; Hsiang-Yi Huang; Fu-Yuan Huang; Chin-Yun Lee; Lung-Ji Chang; T.-C. Wu; Li-Min Huang; and the SARS Research Group of National Taiwan University College of Medicine and National Taiwan University Hospital
- 作者服務機構: 1 Department of Pediatrics and ; ; 2 Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan; ; 3 Department of Pediatrics, Taipei Mackay Memorial Hospital Taipei, Taiwan;; 4 Department of Molecular Genetics and Microbiology, University of Florida, Florida, USA; ; 5 Departments of Pathology, Oncology, Obstetrics and Gynecology and Molecular Microbiology and Immunology, The Johns Hopkins University, Maryland, USA
- 中文摘要: --
- 英文摘要: Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel SARS-associated coronavirus (SARS-CoV). The clinical characteristics are high fever, rapidly progressive diffuse pneumonitis and respiratory distress. It is highly infectious through intimate contact or direct contact with infectious body fluids. Outbreaks within communities and hospitals have been reported. Development of rapid and reliable diagnostic tools is urgently needed. We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using whole virus antigen of SARS-CoV. Eighty-six serum samples collected from patients who were hospitalized for other causes were examined to determine the cut-off O.D. value. The cut-off O.D. value was defined as 0.175 by calculating the mean O.D. value of the 86 sera plus 3 standard deviations. To determine the sensitivity and specificity of the ELISA, 56 positive sera and 204 negative sera were tested. The sensitivity was 96.4% and the specificity was 100%. The results suggest that the IgG ELISA using whole virus antigen of SARS-CoV has a high sensitivity and specificity in detecting SARS IgG antibodies. This IgG ELISA is a powerful tool for serodiagnosis of SARS.
- 中文關鍵字: --
- 英文關鍵字: coronavirus, enzyme-linked immunosorbent assay, serodiagnosis, severe acute respiratory syndrome